Saline:Article Title: The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
Article Snippet: .. The following reagents and instruments were used: Si Jun Zi decoction (Nanjing Hospital of Chinese Medicine), ginsenosideRg1 (CAS No. 22427-39-0, 99.91%, Sigma, St. Louis); Hank Balanced Salt Solution (HBSS; Sbjbio, Nanjing, China), 10% fetal bovine serum (Sbjbio), Trypsin-EDTA Solution (Sbjbio), I-type collagenase (0.2 mg/mL; Thermo Fisher Scientific, Waltham); penicillin/streptomycin, and complete culture medium (Sigma); anti-CD73 (Thermo Fisher Scientific, APC), CD90 (Elabscience, Wuhan, China), CD105 (Thermo Fisher Scientific, FITC) and anti-CD34, CD45 (Elabscience); 1 × phosphate buffer saline (PBS; Gibco, Waltham); Dulbecco modified Eagle medium (DMEM; Gibco); high sensitivity assay ELISA kits (LAPUDA, Nanjing, China); the MDA, SOD, CAT, and GSH assay kits (Njjcbio, Nanjing, China); Anti-RUNX2 antibody, Anti-OPN antibody, Anti-HIF-1α antibody, and Anti-GAPDH antibody (Abcam, Cambridge, UK); flow cytometry (Beckman, Brea); 6-well plates (Costar, Corning); 10 mM β-glycerophosphate, and 2 μM/mL l-glutamine (Sigma); 96-well plates (Corning); microplate reader (Bio-Tek, Winooski); PrimeScriptTM RT Master Mix (Takara Bio, Kusatsu, Shiga, Japan); the Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific); Ultra-high performance liquid chromatography (Thermo Fisher Scientific); The ChemiDoc MP Imaging System (Bio-Rad, Hercules); The low-temperature high-speed centrifuge (Thermo Fisher Scientific). ..
Article Title: The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
Article Snippet: .. The following reagents and instruments were used: Si Jun Zi decoction (Nanjing Hospital of Chinese Medicine), ginsenoside-Rg1 (CAS No. 22427-39-0, 99.91%, Sigma, St. Louis); Hank Balanced Salt Solution (HBSS; Sbjbio, Nanjing, China), 10% fetal bovine serum (Sbjbio), Trypsin-EDTA Solution (Sbjbio), I-type collagenase (0.2 mg/mL; Thermo Fisher Scientific, Waltham); penicillin/streptomycin, and complete culture medium (Sigma); anti-CD73 (Thermo Fisher Scientific, APC), CD90 (Elabscience, Wuhan, China), CD105 (Thermo Fisher Scientific, FITC) and anti-CD34, CD45 (Elabscience); 1 × phosphate buffer saline (PBS; Gibco, Waltham); Dulbecco modified Eagle medium (DMEM; Gibco); high sensitivity assay ELISA kits (LAPUDA, Nanjing, China); the MDA, SOD, CAT, and GSH assay kits (Njjcbio, Nanjing, China); Anti-RUNX2 antibody, Anti-OPN antibody, Anti-HIF-1α antibody, and Anti-GAPDH antibody (Abcam, Cambridge, UK); flow cytometry (Beckman, Brea); 6-well plates (Costar, Corning); 10 mM β-glycerophosphate, and 2 μM/mL l -glutamine (Sigma); 96-well plates (Corning); microplate reader (Bio-Tek, Winooski); PrimeScriptTM RT Master Mix (Takara Bio, Kusatsu, Shiga, Japan); the Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific); Ultra-high performance liquid chromatography (Thermo Fisher Scientific); The ChemiDoc MP Imaging System (Bio-Rad, Hercules); The low-temperature high-speed centrifuge (Thermo Fisher Scientific). ..
Modification:Article Title: The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
Article Snippet: .. The following reagents and instruments were used: Si Jun Zi decoction (Nanjing Hospital of Chinese Medicine), ginsenosideRg1 (CAS No. 22427-39-0, 99.91%, Sigma, St. Louis); Hank Balanced Salt Solution (HBSS; Sbjbio, Nanjing, China), 10% fetal bovine serum (Sbjbio), Trypsin-EDTA Solution (Sbjbio), I-type collagenase (0.2 mg/mL; Thermo Fisher Scientific, Waltham); penicillin/streptomycin, and complete culture medium (Sigma); anti-CD73 (Thermo Fisher Scientific, APC), CD90 (Elabscience, Wuhan, China), CD105 (Thermo Fisher Scientific, FITC) and anti-CD34, CD45 (Elabscience); 1 × phosphate buffer saline (PBS; Gibco, Waltham); Dulbecco modified Eagle medium (DMEM; Gibco); high sensitivity assay ELISA kits (LAPUDA, Nanjing, China); the MDA, SOD, CAT, and GSH assay kits (Njjcbio, Nanjing, China); Anti-RUNX2 antibody, Anti-OPN antibody, Anti-HIF-1α antibody, and Anti-GAPDH antibody (Abcam, Cambridge, UK); flow cytometry (Beckman, Brea); 6-well plates (Costar, Corning); 10 mM β-glycerophosphate, and 2 μM/mL l-glutamine (Sigma); 96-well plates (Corning); microplate reader (Bio-Tek, Winooski); PrimeScriptTM RT Master Mix (Takara Bio, Kusatsu, Shiga, Japan); the Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific); Ultra-high performance liquid chromatography (Thermo Fisher Scientific); The ChemiDoc MP Imaging System (Bio-Rad, Hercules); The low-temperature high-speed centrifuge (Thermo Fisher Scientific). ..
Article Title: The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
Article Snippet: .. The following reagents and instruments were used: Si Jun Zi decoction (Nanjing Hospital of Chinese Medicine), ginsenoside-Rg1 (CAS No. 22427-39-0, 99.91%, Sigma, St. Louis); Hank Balanced Salt Solution (HBSS; Sbjbio, Nanjing, China), 10% fetal bovine serum (Sbjbio), Trypsin-EDTA Solution (Sbjbio), I-type collagenase (0.2 mg/mL; Thermo Fisher Scientific, Waltham); penicillin/streptomycin, and complete culture medium (Sigma); anti-CD73 (Thermo Fisher Scientific, APC), CD90 (Elabscience, Wuhan, China), CD105 (Thermo Fisher Scientific, FITC) and anti-CD34, CD45 (Elabscience); 1 × phosphate buffer saline (PBS; Gibco, Waltham); Dulbecco modified Eagle medium (DMEM; Gibco); high sensitivity assay ELISA kits (LAPUDA, Nanjing, China); the MDA, SOD, CAT, and GSH assay kits (Njjcbio, Nanjing, China); Anti-RUNX2 antibody, Anti-OPN antibody, Anti-HIF-1α antibody, and Anti-GAPDH antibody (Abcam, Cambridge, UK); flow cytometry (Beckman, Brea); 6-well plates (Costar, Corning); 10 mM β-glycerophosphate, and 2 μM/mL l -glutamine (Sigma); 96-well plates (Corning); microplate reader (Bio-Tek, Winooski); PrimeScriptTM RT Master Mix (Takara Bio, Kusatsu, Shiga, Japan); the Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific); Ultra-high performance liquid chromatography (Thermo Fisher Scientific); The ChemiDoc MP Imaging System (Bio-Rad, Hercules); The low-temperature high-speed centrifuge (Thermo Fisher Scientific). ..
Sensitive Assay:Article Title: The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
Article Snippet: .. The following reagents and instruments were used: Si Jun Zi decoction (Nanjing Hospital of Chinese Medicine), ginsenosideRg1 (CAS No. 22427-39-0, 99.91%, Sigma, St. Louis); Hank Balanced Salt Solution (HBSS; Sbjbio, Nanjing, China), 10% fetal bovine serum (Sbjbio), Trypsin-EDTA Solution (Sbjbio), I-type collagenase (0.2 mg/mL; Thermo Fisher Scientific, Waltham); penicillin/streptomycin, and complete culture medium (Sigma); anti-CD73 (Thermo Fisher Scientific, APC), CD90 (Elabscience, Wuhan, China), CD105 (Thermo Fisher Scientific, FITC) and anti-CD34, CD45 (Elabscience); 1 × phosphate buffer saline (PBS; Gibco, Waltham); Dulbecco modified Eagle medium (DMEM; Gibco); high sensitivity assay ELISA kits (LAPUDA, Nanjing, China); the MDA, SOD, CAT, and GSH assay kits (Njjcbio, Nanjing, China); Anti-RUNX2 antibody, Anti-OPN antibody, Anti-HIF-1α antibody, and Anti-GAPDH antibody (Abcam, Cambridge, UK); flow cytometry (Beckman, Brea); 6-well plates (Costar, Corning); 10 mM β-glycerophosphate, and 2 μM/mL l-glutamine (Sigma); 96-well plates (Corning); microplate reader (Bio-Tek, Winooski); PrimeScriptTM RT Master Mix (Takara Bio, Kusatsu, Shiga, Japan); the Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific); Ultra-high performance liquid chromatography (Thermo Fisher Scientific); The ChemiDoc MP Imaging System (Bio-Rad, Hercules); The low-temperature high-speed centrifuge (Thermo Fisher Scientific). ..
Article Title: The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
Article Snippet: .. The following reagents and instruments were used: Si Jun Zi decoction (Nanjing Hospital of Chinese Medicine), ginsenoside-Rg1 (CAS No. 22427-39-0, 99.91%, Sigma, St. Louis); Hank Balanced Salt Solution (HBSS; Sbjbio, Nanjing, China), 10% fetal bovine serum (Sbjbio), Trypsin-EDTA Solution (Sbjbio), I-type collagenase (0.2 mg/mL; Thermo Fisher Scientific, Waltham); penicillin/streptomycin, and complete culture medium (Sigma); anti-CD73 (Thermo Fisher Scientific, APC), CD90 (Elabscience, Wuhan, China), CD105 (Thermo Fisher Scientific, FITC) and anti-CD34, CD45 (Elabscience); 1 × phosphate buffer saline (PBS; Gibco, Waltham); Dulbecco modified Eagle medium (DMEM; Gibco); high sensitivity assay ELISA kits (LAPUDA, Nanjing, China); the MDA, SOD, CAT, and GSH assay kits (Njjcbio, Nanjing, China); Anti-RUNX2 antibody, Anti-OPN antibody, Anti-HIF-1α antibody, and Anti-GAPDH antibody (Abcam, Cambridge, UK); flow cytometry (Beckman, Brea); 6-well plates (Costar, Corning); 10 mM β-glycerophosphate, and 2 μM/mL l -glutamine (Sigma); 96-well plates (Corning); microplate reader (Bio-Tek, Winooski); PrimeScriptTM RT Master Mix (Takara Bio, Kusatsu, Shiga, Japan); the Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific); Ultra-high performance liquid chromatography (Thermo Fisher Scientific); The ChemiDoc MP Imaging System (Bio-Rad, Hercules); The low-temperature high-speed centrifuge (Thermo Fisher Scientific). ..
Enzyme-linked Immunosorbent Assay:Article Title: The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
Article Snippet: .. The following reagents and instruments were used: Si Jun Zi decoction (Nanjing Hospital of Chinese Medicine), ginsenosideRg1 (CAS No. 22427-39-0, 99.91%, Sigma, St. Louis); Hank Balanced Salt Solution (HBSS; Sbjbio, Nanjing, China), 10% fetal bovine serum (Sbjbio), Trypsin-EDTA Solution (Sbjbio), I-type collagenase (0.2 mg/mL; Thermo Fisher Scientific, Waltham); penicillin/streptomycin, and complete culture medium (Sigma); anti-CD73 (Thermo Fisher Scientific, APC), CD90 (Elabscience, Wuhan, China), CD105 (Thermo Fisher Scientific, FITC) and anti-CD34, CD45 (Elabscience); 1 × phosphate buffer saline (PBS; Gibco, Waltham); Dulbecco modified Eagle medium (DMEM; Gibco); high sensitivity assay ELISA kits (LAPUDA, Nanjing, China); the MDA, SOD, CAT, and GSH assay kits (Njjcbio, Nanjing, China); Anti-RUNX2 antibody, Anti-OPN antibody, Anti-HIF-1α antibody, and Anti-GAPDH antibody (Abcam, Cambridge, UK); flow cytometry (Beckman, Brea); 6-well plates (Costar, Corning); 10 mM β-glycerophosphate, and 2 μM/mL l-glutamine (Sigma); 96-well plates (Corning); microplate reader (Bio-Tek, Winooski); PrimeScriptTM RT Master Mix (Takara Bio, Kusatsu, Shiga, Japan); the Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific); Ultra-high performance liquid chromatography (Thermo Fisher Scientific); The ChemiDoc MP Imaging System (Bio-Rad, Hercules); The low-temperature high-speed centrifuge (Thermo Fisher Scientific). ..
Article Title: The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
Article Snippet: .. The following reagents and instruments were used: Si Jun Zi decoction (Nanjing Hospital of Chinese Medicine), ginsenoside-Rg1 (CAS No. 22427-39-0, 99.91%, Sigma, St. Louis); Hank Balanced Salt Solution (HBSS; Sbjbio, Nanjing, China), 10% fetal bovine serum (Sbjbio), Trypsin-EDTA Solution (Sbjbio), I-type collagenase (0.2 mg/mL; Thermo Fisher Scientific, Waltham); penicillin/streptomycin, and complete culture medium (Sigma); anti-CD73 (Thermo Fisher Scientific, APC), CD90 (Elabscience, Wuhan, China), CD105 (Thermo Fisher Scientific, FITC) and anti-CD34, CD45 (Elabscience); 1 × phosphate buffer saline (PBS; Gibco, Waltham); Dulbecco modified Eagle medium (DMEM; Gibco); high sensitivity assay ELISA kits (LAPUDA, Nanjing, China); the MDA, SOD, CAT, and GSH assay kits (Njjcbio, Nanjing, China); Anti-RUNX2 antibody, Anti-OPN antibody, Anti-HIF-1α antibody, and Anti-GAPDH antibody (Abcam, Cambridge, UK); flow cytometry (Beckman, Brea); 6-well plates (Costar, Corning); 10 mM β-glycerophosphate, and 2 μM/mL l -glutamine (Sigma); 96-well plates (Corning); microplate reader (Bio-Tek, Winooski); PrimeScriptTM RT Master Mix (Takara Bio, Kusatsu, Shiga, Japan); the Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific); Ultra-high performance liquid chromatography (Thermo Fisher Scientific); The ChemiDoc MP Imaging System (Bio-Rad, Hercules); The low-temperature high-speed centrifuge (Thermo Fisher Scientific). ..
Multiple Displacement Amplification:Article Title: The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
Article Snippet: .. The following reagents and instruments were used: Si Jun Zi decoction (Nanjing Hospital of Chinese Medicine), ginsenosideRg1 (CAS No. 22427-39-0, 99.91%, Sigma, St. Louis); Hank Balanced Salt Solution (HBSS; Sbjbio, Nanjing, China), 10% fetal bovine serum (Sbjbio), Trypsin-EDTA Solution (Sbjbio), I-type collagenase (0.2 mg/mL; Thermo Fisher Scientific, Waltham); penicillin/streptomycin, and complete culture medium (Sigma); anti-CD73 (Thermo Fisher Scientific, APC), CD90 (Elabscience, Wuhan, China), CD105 (Thermo Fisher Scientific, FITC) and anti-CD34, CD45 (Elabscience); 1 × phosphate buffer saline (PBS; Gibco, Waltham); Dulbecco modified Eagle medium (DMEM; Gibco); high sensitivity assay ELISA kits (LAPUDA, Nanjing, China); the MDA, SOD, CAT, and GSH assay kits (Njjcbio, Nanjing, China); Anti-RUNX2 antibody, Anti-OPN antibody, Anti-HIF-1α antibody, and Anti-GAPDH antibody (Abcam, Cambridge, UK); flow cytometry (Beckman, Brea); 6-well plates (Costar, Corning); 10 mM β-glycerophosphate, and 2 μM/mL l-glutamine (Sigma); 96-well plates (Corning); microplate reader (Bio-Tek, Winooski); PrimeScriptTM RT Master Mix (Takara Bio, Kusatsu, Shiga, Japan); the Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific); Ultra-high performance liquid chromatography (Thermo Fisher Scientific); The ChemiDoc MP Imaging System (Bio-Rad, Hercules); The low-temperature high-speed centrifuge (Thermo Fisher Scientific). ..
Article Title: The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
Article Snippet: .. The following reagents and instruments were used: Si Jun Zi decoction (Nanjing Hospital of Chinese Medicine), ginsenoside-Rg1 (CAS No. 22427-39-0, 99.91%, Sigma, St. Louis); Hank Balanced Salt Solution (HBSS; Sbjbio, Nanjing, China), 10% fetal bovine serum (Sbjbio), Trypsin-EDTA Solution (Sbjbio), I-type collagenase (0.2 mg/mL; Thermo Fisher Scientific, Waltham); penicillin/streptomycin, and complete culture medium (Sigma); anti-CD73 (Thermo Fisher Scientific, APC), CD90 (Elabscience, Wuhan, China), CD105 (Thermo Fisher Scientific, FITC) and anti-CD34, CD45 (Elabscience); 1 × phosphate buffer saline (PBS; Gibco, Waltham); Dulbecco modified Eagle medium (DMEM; Gibco); high sensitivity assay ELISA kits (LAPUDA, Nanjing, China); the MDA, SOD, CAT, and GSH assay kits (Njjcbio, Nanjing, China); Anti-RUNX2 antibody, Anti-OPN antibody, Anti-HIF-1α antibody, and Anti-GAPDH antibody (Abcam, Cambridge, UK); flow cytometry (Beckman, Brea); 6-well plates (Costar, Corning); 10 mM β-glycerophosphate, and 2 μM/mL l -glutamine (Sigma); 96-well plates (Corning); microplate reader (Bio-Tek, Winooski); PrimeScriptTM RT Master Mix (Takara Bio, Kusatsu, Shiga, Japan); the Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific); Ultra-high performance liquid chromatography (Thermo Fisher Scientific); The ChemiDoc MP Imaging System (Bio-Rad, Hercules); The low-temperature high-speed centrifuge (Thermo Fisher Scientific). ..
GSH Assay:Article Title: The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
Article Snippet: .. The following reagents and instruments were used: Si Jun Zi decoction (Nanjing Hospital of Chinese Medicine), ginsenosideRg1 (CAS No. 22427-39-0, 99.91%, Sigma, St. Louis); Hank Balanced Salt Solution (HBSS; Sbjbio, Nanjing, China), 10% fetal bovine serum (Sbjbio), Trypsin-EDTA Solution (Sbjbio), I-type collagenase (0.2 mg/mL; Thermo Fisher Scientific, Waltham); penicillin/streptomycin, and complete culture medium (Sigma); anti-CD73 (Thermo Fisher Scientific, APC), CD90 (Elabscience, Wuhan, China), CD105 (Thermo Fisher Scientific, FITC) and anti-CD34, CD45 (Elabscience); 1 × phosphate buffer saline (PBS; Gibco, Waltham); Dulbecco modified Eagle medium (DMEM; Gibco); high sensitivity assay ELISA kits (LAPUDA, Nanjing, China); the MDA, SOD, CAT, and GSH assay kits (Njjcbio, Nanjing, China); Anti-RUNX2 antibody, Anti-OPN antibody, Anti-HIF-1α antibody, and Anti-GAPDH antibody (Abcam, Cambridge, UK); flow cytometry (Beckman, Brea); 6-well plates (Costar, Corning); 10 mM β-glycerophosphate, and 2 μM/mL l-glutamine (Sigma); 96-well plates (Corning); microplate reader (Bio-Tek, Winooski); PrimeScriptTM RT Master Mix (Takara Bio, Kusatsu, Shiga, Japan); the Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific); Ultra-high performance liquid chromatography (Thermo Fisher Scientific); The ChemiDoc MP Imaging System (Bio-Rad, Hercules); The low-temperature high-speed centrifuge (Thermo Fisher Scientific). ..
Article Title: The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
Article Snippet: .. The following reagents and instruments were used: Si Jun Zi decoction (Nanjing Hospital of Chinese Medicine), ginsenoside-Rg1 (CAS No. 22427-39-0, 99.91%, Sigma, St. Louis); Hank Balanced Salt Solution (HBSS; Sbjbio, Nanjing, China), 10% fetal bovine serum (Sbjbio), Trypsin-EDTA Solution (Sbjbio), I-type collagenase (0.2 mg/mL; Thermo Fisher Scientific, Waltham); penicillin/streptomycin, and complete culture medium (Sigma); anti-CD73 (Thermo Fisher Scientific, APC), CD90 (Elabscience, Wuhan, China), CD105 (Thermo Fisher Scientific, FITC) and anti-CD34, CD45 (Elabscience); 1 × phosphate buffer saline (PBS; Gibco, Waltham); Dulbecco modified Eagle medium (DMEM; Gibco); high sensitivity assay ELISA kits (LAPUDA, Nanjing, China); the MDA, SOD, CAT, and GSH assay kits (Njjcbio, Nanjing, China); Anti-RUNX2 antibody, Anti-OPN antibody, Anti-HIF-1α antibody, and Anti-GAPDH antibody (Abcam, Cambridge, UK); flow cytometry (Beckman, Brea); 6-well plates (Costar, Corning); 10 mM β-glycerophosphate, and 2 μM/mL l -glutamine (Sigma); 96-well plates (Corning); microplate reader (Bio-Tek, Winooski); PrimeScriptTM RT Master Mix (Takara Bio, Kusatsu, Shiga, Japan); the Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific); Ultra-high performance liquid chromatography (Thermo Fisher Scientific); The ChemiDoc MP Imaging System (Bio-Rad, Hercules); The low-temperature high-speed centrifuge (Thermo Fisher Scientific). ..
Flow Cytometry:Article Title: The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
Article Snippet: .. The following reagents and instruments were used: Si Jun Zi decoction (Nanjing Hospital of Chinese Medicine), ginsenosideRg1 (CAS No. 22427-39-0, 99.91%, Sigma, St. Louis); Hank Balanced Salt Solution (HBSS; Sbjbio, Nanjing, China), 10% fetal bovine serum (Sbjbio), Trypsin-EDTA Solution (Sbjbio), I-type collagenase (0.2 mg/mL; Thermo Fisher Scientific, Waltham); penicillin/streptomycin, and complete culture medium (Sigma); anti-CD73 (Thermo Fisher Scientific, APC), CD90 (Elabscience, Wuhan, China), CD105 (Thermo Fisher Scientific, FITC) and anti-CD34, CD45 (Elabscience); 1 × phosphate buffer saline (PBS; Gibco, Waltham); Dulbecco modified Eagle medium (DMEM; Gibco); high sensitivity assay ELISA kits (LAPUDA, Nanjing, China); the MDA, SOD, CAT, and GSH assay kits (Njjcbio, Nanjing, China); Anti-RUNX2 antibody, Anti-OPN antibody, Anti-HIF-1α antibody, and Anti-GAPDH antibody (Abcam, Cambridge, UK); flow cytometry (Beckman, Brea); 6-well plates (Costar, Corning); 10 mM β-glycerophosphate, and 2 μM/mL l-glutamine (Sigma); 96-well plates (Corning); microplate reader (Bio-Tek, Winooski); PrimeScriptTM RT Master Mix (Takara Bio, Kusatsu, Shiga, Japan); the Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific); Ultra-high performance liquid chromatography (Thermo Fisher Scientific); The ChemiDoc MP Imaging System (Bio-Rad, Hercules); The low-temperature high-speed centrifuge (Thermo Fisher Scientific). ..
Article Title: The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
Article Snippet: .. The following reagents and instruments were used: Si Jun Zi decoction (Nanjing Hospital of Chinese Medicine), ginsenoside-Rg1 (CAS No. 22427-39-0, 99.91%, Sigma, St. Louis); Hank Balanced Salt Solution (HBSS; Sbjbio, Nanjing, China), 10% fetal bovine serum (Sbjbio), Trypsin-EDTA Solution (Sbjbio), I-type collagenase (0.2 mg/mL; Thermo Fisher Scientific, Waltham); penicillin/streptomycin, and complete culture medium (Sigma); anti-CD73 (Thermo Fisher Scientific, APC), CD90 (Elabscience, Wuhan, China), CD105 (Thermo Fisher Scientific, FITC) and anti-CD34, CD45 (Elabscience); 1 × phosphate buffer saline (PBS; Gibco, Waltham); Dulbecco modified Eagle medium (DMEM; Gibco); high sensitivity assay ELISA kits (LAPUDA, Nanjing, China); the MDA, SOD, CAT, and GSH assay kits (Njjcbio, Nanjing, China); Anti-RUNX2 antibody, Anti-OPN antibody, Anti-HIF-1α antibody, and Anti-GAPDH antibody (Abcam, Cambridge, UK); flow cytometry (Beckman, Brea); 6-well plates (Costar, Corning); 10 mM β-glycerophosphate, and 2 μM/mL l -glutamine (Sigma); 96-well plates (Corning); microplate reader (Bio-Tek, Winooski); PrimeScriptTM RT Master Mix (Takara Bio, Kusatsu, Shiga, Japan); the Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific); Ultra-high performance liquid chromatography (Thermo Fisher Scientific); The ChemiDoc MP Imaging System (Bio-Rad, Hercules); The low-temperature high-speed centrifuge (Thermo Fisher Scientific). ..
Real-time Polymerase Chain Reaction:Article Title: The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
Article Snippet: .. The following reagents and instruments were used: Si Jun Zi decoction (Nanjing Hospital of Chinese Medicine), ginsenosideRg1 (CAS No. 22427-39-0, 99.91%, Sigma, St. Louis); Hank Balanced Salt Solution (HBSS; Sbjbio, Nanjing, China), 10% fetal bovine serum (Sbjbio), Trypsin-EDTA Solution (Sbjbio), I-type collagenase (0.2 mg/mL; Thermo Fisher Scientific, Waltham); penicillin/streptomycin, and complete culture medium (Sigma); anti-CD73 (Thermo Fisher Scientific, APC), CD90 (Elabscience, Wuhan, China), CD105 (Thermo Fisher Scientific, FITC) and anti-CD34, CD45 (Elabscience); 1 × phosphate buffer saline (PBS; Gibco, Waltham); Dulbecco modified Eagle medium (DMEM; Gibco); high sensitivity assay ELISA kits (LAPUDA, Nanjing, China); the MDA, SOD, CAT, and GSH assay kits (Njjcbio, Nanjing, China); Anti-RUNX2 antibody, Anti-OPN antibody, Anti-HIF-1α antibody, and Anti-GAPDH antibody (Abcam, Cambridge, UK); flow cytometry (Beckman, Brea); 6-well plates (Costar, Corning); 10 mM β-glycerophosphate, and 2 μM/mL l-glutamine (Sigma); 96-well plates (Corning); microplate reader (Bio-Tek, Winooski); PrimeScriptTM RT Master Mix (Takara Bio, Kusatsu, Shiga, Japan); the Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific); Ultra-high performance liquid chromatography (Thermo Fisher Scientific); The ChemiDoc MP Imaging System (Bio-Rad, Hercules); The low-temperature high-speed centrifuge (Thermo Fisher Scientific). ..
Article Title: The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
Article Snippet: .. The following reagents and instruments were used: Si Jun Zi decoction (Nanjing Hospital of Chinese Medicine), ginsenoside-Rg1 (CAS No. 22427-39-0, 99.91%, Sigma, St. Louis); Hank Balanced Salt Solution (HBSS; Sbjbio, Nanjing, China), 10% fetal bovine serum (Sbjbio), Trypsin-EDTA Solution (Sbjbio), I-type collagenase (0.2 mg/mL; Thermo Fisher Scientific, Waltham); penicillin/streptomycin, and complete culture medium (Sigma); anti-CD73 (Thermo Fisher Scientific, APC), CD90 (Elabscience, Wuhan, China), CD105 (Thermo Fisher Scientific, FITC) and anti-CD34, CD45 (Elabscience); 1 × phosphate buffer saline (PBS; Gibco, Waltham); Dulbecco modified Eagle medium (DMEM; Gibco); high sensitivity assay ELISA kits (LAPUDA, Nanjing, China); the MDA, SOD, CAT, and GSH assay kits (Njjcbio, Nanjing, China); Anti-RUNX2 antibody, Anti-OPN antibody, Anti-HIF-1α antibody, and Anti-GAPDH antibody (Abcam, Cambridge, UK); flow cytometry (Beckman, Brea); 6-well plates (Costar, Corning); 10 mM β-glycerophosphate, and 2 μM/mL l -glutamine (Sigma); 96-well plates (Corning); microplate reader (Bio-Tek, Winooski); PrimeScriptTM RT Master Mix (Takara Bio, Kusatsu, Shiga, Japan); the Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific); Ultra-high performance liquid chromatography (Thermo Fisher Scientific); The ChemiDoc MP Imaging System (Bio-Rad, Hercules); The low-temperature high-speed centrifuge (Thermo Fisher Scientific). ..
Liquid Chromatography:Article Title: The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
Article Snippet: .. The following reagents and instruments were used: Si Jun Zi decoction (Nanjing Hospital of Chinese Medicine), ginsenosideRg1 (CAS No. 22427-39-0, 99.91%, Sigma, St. Louis); Hank Balanced Salt Solution (HBSS; Sbjbio, Nanjing, China), 10% fetal bovine serum (Sbjbio), Trypsin-EDTA Solution (Sbjbio), I-type collagenase (0.2 mg/mL; Thermo Fisher Scientific, Waltham); penicillin/streptomycin, and complete culture medium (Sigma); anti-CD73 (Thermo Fisher Scientific, APC), CD90 (Elabscience, Wuhan, China), CD105 (Thermo Fisher Scientific, FITC) and anti-CD34, CD45 (Elabscience); 1 × phosphate buffer saline (PBS; Gibco, Waltham); Dulbecco modified Eagle medium (DMEM; Gibco); high sensitivity assay ELISA kits (LAPUDA, Nanjing, China); the MDA, SOD, CAT, and GSH assay kits (Njjcbio, Nanjing, China); Anti-RUNX2 antibody, Anti-OPN antibody, Anti-HIF-1α antibody, and Anti-GAPDH antibody (Abcam, Cambridge, UK); flow cytometry (Beckman, Brea); 6-well plates (Costar, Corning); 10 mM β-glycerophosphate, and 2 μM/mL l-glutamine (Sigma); 96-well plates (Corning); microplate reader (Bio-Tek, Winooski); PrimeScriptTM RT Master Mix (Takara Bio, Kusatsu, Shiga, Japan); the Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific); Ultra-high performance liquid chromatography (Thermo Fisher Scientific); The ChemiDoc MP Imaging System (Bio-Rad, Hercules); The low-temperature high-speed centrifuge (Thermo Fisher Scientific). ..
Article Title: The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
Article Snippet: .. The following reagents and instruments were used: Si Jun Zi decoction (Nanjing Hospital of Chinese Medicine), ginsenoside-Rg1 (CAS No. 22427-39-0, 99.91%, Sigma, St. Louis); Hank Balanced Salt Solution (HBSS; Sbjbio, Nanjing, China), 10% fetal bovine serum (Sbjbio), Trypsin-EDTA Solution (Sbjbio), I-type collagenase (0.2 mg/mL; Thermo Fisher Scientific, Waltham); penicillin/streptomycin, and complete culture medium (Sigma); anti-CD73 (Thermo Fisher Scientific, APC), CD90 (Elabscience, Wuhan, China), CD105 (Thermo Fisher Scientific, FITC) and anti-CD34, CD45 (Elabscience); 1 × phosphate buffer saline (PBS; Gibco, Waltham); Dulbecco modified Eagle medium (DMEM; Gibco); high sensitivity assay ELISA kits (LAPUDA, Nanjing, China); the MDA, SOD, CAT, and GSH assay kits (Njjcbio, Nanjing, China); Anti-RUNX2 antibody, Anti-OPN antibody, Anti-HIF-1α antibody, and Anti-GAPDH antibody (Abcam, Cambridge, UK); flow cytometry (Beckman, Brea); 6-well plates (Costar, Corning); 10 mM β-glycerophosphate, and 2 μM/mL l -glutamine (Sigma); 96-well plates (Corning); microplate reader (Bio-Tek, Winooski); PrimeScriptTM RT Master Mix (Takara Bio, Kusatsu, Shiga, Japan); the Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific); Ultra-high performance liquid chromatography (Thermo Fisher Scientific); The ChemiDoc MP Imaging System (Bio-Rad, Hercules); The low-temperature high-speed centrifuge (Thermo Fisher Scientific). ..
Imaging:Article Title: The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
Article Snippet: .. The following reagents and instruments were used: Si Jun Zi decoction (Nanjing Hospital of Chinese Medicine), ginsenosideRg1 (CAS No. 22427-39-0, 99.91%, Sigma, St. Louis); Hank Balanced Salt Solution (HBSS; Sbjbio, Nanjing, China), 10% fetal bovine serum (Sbjbio), Trypsin-EDTA Solution (Sbjbio), I-type collagenase (0.2 mg/mL; Thermo Fisher Scientific, Waltham); penicillin/streptomycin, and complete culture medium (Sigma); anti-CD73 (Thermo Fisher Scientific, APC), CD90 (Elabscience, Wuhan, China), CD105 (Thermo Fisher Scientific, FITC) and anti-CD34, CD45 (Elabscience); 1 × phosphate buffer saline (PBS; Gibco, Waltham); Dulbecco modified Eagle medium (DMEM; Gibco); high sensitivity assay ELISA kits (LAPUDA, Nanjing, China); the MDA, SOD, CAT, and GSH assay kits (Njjcbio, Nanjing, China); Anti-RUNX2 antibody, Anti-OPN antibody, Anti-HIF-1α antibody, and Anti-GAPDH antibody (Abcam, Cambridge, UK); flow cytometry (Beckman, Brea); 6-well plates (Costar, Corning); 10 mM β-glycerophosphate, and 2 μM/mL l-glutamine (Sigma); 96-well plates (Corning); microplate reader (Bio-Tek, Winooski); PrimeScriptTM RT Master Mix (Takara Bio, Kusatsu, Shiga, Japan); the Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific); Ultra-high performance liquid chromatography (Thermo Fisher Scientific); The ChemiDoc MP Imaging System (Bio-Rad, Hercules); The low-temperature high-speed centrifuge (Thermo Fisher Scientific). ..
Article Title: The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
Article Snippet: .. The following reagents and instruments were used: Si Jun Zi decoction (Nanjing Hospital of Chinese Medicine), ginsenoside-Rg1 (CAS No. 22427-39-0, 99.91%, Sigma, St. Louis); Hank Balanced Salt Solution (HBSS; Sbjbio, Nanjing, China), 10% fetal bovine serum (Sbjbio), Trypsin-EDTA Solution (Sbjbio), I-type collagenase (0.2 mg/mL; Thermo Fisher Scientific, Waltham); penicillin/streptomycin, and complete culture medium (Sigma); anti-CD73 (Thermo Fisher Scientific, APC), CD90 (Elabscience, Wuhan, China), CD105 (Thermo Fisher Scientific, FITC) and anti-CD34, CD45 (Elabscience); 1 × phosphate buffer saline (PBS; Gibco, Waltham); Dulbecco modified Eagle medium (DMEM; Gibco); high sensitivity assay ELISA kits (LAPUDA, Nanjing, China); the MDA, SOD, CAT, and GSH assay kits (Njjcbio, Nanjing, China); Anti-RUNX2 antibody, Anti-OPN antibody, Anti-HIF-1α antibody, and Anti-GAPDH antibody (Abcam, Cambridge, UK); flow cytometry (Beckman, Brea); 6-well plates (Costar, Corning); 10 mM β-glycerophosphate, and 2 μM/mL l -glutamine (Sigma); 96-well plates (Corning); microplate reader (Bio-Tek, Winooski); PrimeScriptTM RT Master Mix (Takara Bio, Kusatsu, Shiga, Japan); the Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific); Ultra-high performance liquid chromatography (Thermo Fisher Scientific); The ChemiDoc MP Imaging System (Bio-Rad, Hercules); The low-temperature high-speed centrifuge (Thermo Fisher Scientific). ..
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